Research Paper Volume 13, Issue 20 pp 23757—23768

Circular RNA circPRKCI contributes to malignant progression of T-cell acute lymphoblastic leukemia by modulating miR-20a-5p/SOX4 axis

CircPRKCI serves as a ceRNA of miR-20a-5p in T-ALL cells. (A) The predicted interaction site between circPRKCI and miR-20a-5p. (B–D) MOLT-4 and JURKAT cells were treated with miR-20a-5p mimic. (B) The treatment efficacy of miR-20a-5p mimic in MOLT-4 and JURKAT cells was determined by qRT-PCR assay. (C, D) The luciferase activity of circPRKCI was determined by dual luciferase reporter assay. (E, F) MOLT-4 and JURKAT cells were treated with circPRKCI shRNA. The expression of miR-20a-5p was examined by qRT-PCR assay. **p

Figure 3. CircPRKCI serves as a ceRNA of miR-20a-5p in T-ALL cells. (A) The predicted interaction site between circPRKCI and miR-20a-5p. (BD) MOLT-4 and JURKAT cells were treated with miR-20a-5p mimic. (B) The treatment efficacy of miR-20a-5p mimic in MOLT-4 and JURKAT cells was determined by qRT-PCR assay. (C, D) The luciferase activity of circPRKCI was determined by dual luciferase reporter assay. (E, F) MOLT-4 and JURKAT cells were treated with circPRKCI shRNA. The expression of miR-20a-5p was examined by qRT-PCR assay. **p < 0.01.