Research Paper Volume 13, Issue 20 pp 23769—23779

Hypoxic tubular epithelial cells regulate the angiogenesis of HMEC-1 cells via mediation of Rab7/MMP-2 axis

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Figure 2.

Knockdown of Rab7 reversed hypoxia-induced inhibition of HMEC-1 cell proliferation. In order to detect the function of Rab7 in hypoxia-treated HK-2 cells, cells were cultured in hypoxia and normoxia, respectively. Then, the cell supernatant was collected and cultured in conditioned medium (CM). After that, HMEC-1 cells were maintained in CM. (A) CCK-8 assay was performed to detect the viability of HMEC-1 cells. (B) The proliferation of HMEC-1 cells was tested by EdU staining. (C, D) The apoptosis of HMEC-1 cells was tested by flow cytometry. (E) The protein levels of Bax and cleaved caspase 3 in HMEC-1 cells were detected by western blot. (F, G) β-actin was used for quantification. *P<0.05, **P<0.01.