Research Paper Volume 13, Issue 20 pp 23769—23779

Hypoxic tubular epithelial cells regulate the angiogenesis of HMEC-1 cells via mediation of Rab7/MMP-2 axis

Rab7 shRNA1 reversed hypoxia-induced decrease of migration and angiogenesis in HMEC-1 cells via upregulation of MMP-2 activity. (A, B) The MMP-2 activity in HMEC-1 cells was tested by Gelatin Zymography. (C) The viability of HMEC-1 cells was tested by CCK-8 assay. (D) The migration of HMEC-1 cells was tested by transwell assay. (E) The angiogenesis of HMEC-1 cells was investigating by calculating the total length. **P

Figure 5. Rab7 shRNA1 reversed hypoxia-induced decrease of migration and angiogenesis in HMEC-1 cells via upregulation of MMP-2 activity. (A, B) The MMP-2 activity in HMEC-1 cells was tested by Gelatin Zymography. (C) The viability of HMEC-1 cells was tested by CCK-8 assay. (D) The migration of HMEC-1 cells was tested by transwell assay. (E) The angiogenesis of HMEC-1 cells was investigating by calculating the total length. **P<0.01 compared to normoxia.