Research Paper Volume 13, Issue 20 pp 23780—23795

Matairesinol exerts anti-inflammatory and antioxidant effects in sepsis-mediated brain injury by repressing the MAPK and NF-κB pathways through up-regulating AMPK

Mat facilitated the AMPK expression. In addition to LPS (1 μg/mL) treatment for 24 hours, NSC-34, HT22 and BV2 cells were intervened with Mat (10, 20 μM) for 48 hours. (A–C) The AMPK expression at the mRNA level was examined by qRT-PCR. (D–F) The AMPK protein profile was verified by WB. Rats were orally administered with Mat (10, 20 mg/kg) following CLP treatment. (G) The AMPK expression in tissues was monitored by IHC. (H) The AMPK phosphorylation was measured by WB. **P ***P #P ##P ##P

Figure 6. Mat facilitated the AMPK expression. In addition to LPS (1 μg/mL) treatment for 24 hours, NSC-34, HT22 and BV2 cells were intervened with Mat (10, 20 μM) for 48 hours. (AC) The AMPK expression at the mRNA level was examined by qRT-PCR. (DF) The AMPK protein profile was verified by WB. Rats were orally administered with Mat (10, 20 mg/kg) following CLP treatment. (G) The AMPK expression in tissues was monitored by IHC. (H) The AMPK phosphorylation was measured by WB. **P < 0.01, ***P < 0.001 (vs. control group). #P < 0.05, ##P < 0.01, ##P < 0.001 (vs. LPS group).