Research Paper Volume 13, Issue 20 pp 23780—23795

Figure 7. Attenuating AMPK abated Mat-mediated neuron-protective and anti-inflammatory effects. NSC-34 and BV2 cells were treated with Mat (20 μM) alone or in combination with Compound C for 48 hours following LPS (1 μg/mL) treatment for 24 hours. (A–B) proliferation and apoptosis of NSC-34 cells were measured by CCK8. (C) The expression of Caspase-3 and Bcl2 was tested by WB. (D–G) Detection of MDA, SOD, CAT and GSH-Px levels in NSC-34 was performed using the BCA protein assay kit. (H) ELISA was applied for gauging the production of TNF-α, IL-1β, IL-6, IFN-γ, IL-8 and MCP1 in NSC-34. (I–J) The expression of MAPK and JNK, as well as NF-κB in BV2 microglia, was compared by WB. ^**P < 0.01, ^***P < 0.001 (vs. control group). ^##P < 0.01, ^##P < 0.001 (vs. LPS group). ⁺P < 0.05, ⁺⁺P < 0.01 (vs. LPS+Mat group).