Figure 7. LY294002 inhibits OPN-mediated subchondral bone and cartilage degeneration in OA. (A) Western blot analysis of the expression of PTEN, p-AKT and OPN in MC3T3-E1 cells transfected with PTEN-siRNA and treated with LY294002 (10 μM) for 48 h. (B) Representative images and quantitative analysis of p-AKT and OPN co-immunostaining in the tibial subchondral bone in sham group, OA group and LY294002 treatment group. Positive cells were indicated with arrows. Boxed area is magnified in the corner. Scale bars = 50 μm, n = 6.