Research Paper Volume 13, Issue 24 pp 25778—25798

Figure 9. PKMYT1 promotes the proliferation, migration, and invasion of renal cancer cells in vitro. The mRNA expression of PKMYT1 in ACHN (A) and Caki-1 cells (B) was measured using real-time quantitative reverse transcription-PCR. The protein expression of PKMYT1 in ACHN (C) and Caki-1 cells (D) by western blotting assays. Cell Counting Kit-8 assays were used to detect the effect of PKMYT1 knockdown on cell proliferation in ACHN (E) and Caki-1 (F) cells. Representative images of migration and invasion assays for ACHN (G) and Caki-1 (H) cells. Transwell migration and invasion assays indicated that the migration and invasion abilities of PKMYT1 were weakened in siRNA groups for ACHN (I) and Caki-1 (J) cells. Data are shown as the mean ± standard deviation from three independent experiments, and were compared to the respective si-NC group. ^****P < 0.0001; ^***P < 0.001; ^**P < 0.01 and ^*P < 0.05.