Research Paper Volume 13, Issue 24 pp 25903—25919

METTL14 benefits the mesenchymal stem cells in patients with steroid-associated osteonecrosis of the femoral head by regulating the m6A level of PTPN6


Figure 3. METTL14 up-regulation promoted the proliferation and osteogenesis of SONFH BMSCs. (A) The effect of METTL14 up-regulation on the cell viability of SONFH BMSCs was analyzed by CCK-8 assay. (B) EdU assay was used to detect the influence of METTL14 overexpression on the proliferation of SONFH BMSCs. Scale bar = 100 μm. (C) The ALP activity assay provided evidence that METTL14 up-regulation enhanced the ability of osteogenic differentiation of BMSCs from SONFH patients. (D) METTL14 overexpression promoted the ability of osteogenic differentiation and suppressed the adipogenesis capacity of SONFH BMSCs were confirmed by alizarin red staining and oil red staining. Scale bar = 100 μm. (E) The expression of osteogenic-related and adipogenic-related genes in BMSCs with METTL14 overexpression were analyzed by western blot. *P < 0.05, **P < 0.01, ***P < 0.001, n.s is no significance.