Research Paper Volume 13, Issue 24 pp 26022—26033

LINC00839 knockdown restrains the metastatic behavior of nasopharyngeal carcinoma by sponging miR-454-3p


Figure 1. The expression and effect of LINC00839 on cell growth in NPC. (A, B) The differential gene expression in GSE53819 and GSE64634 data subsets was visualized using volcano plots. (C) Venn diagrams show the intersection of GSE53819 and GSE64634 data subsets. (D) qPCR examined LINC00839 expression in NPC cell lines (SUNE-1, CNE-1, C666-1 and CNE-2) compared with that in normal nasopharyngeal epidermal cell line NP-69. **P < 0.01 compared with NP-69. (E) qPCR analysis testified LINC00839 expression in C666-1 and SUNE-1 cells after transfected with shRNA against LINC00839 (sh-LINC00839 # or sh-LINC00839 #2) or scrambled shRNA (sh-Ctrl). (F, G) After transfection, cell proliferation was determined by MTT assay. (H) The growth of NPC cells was determined by colony formation assay. **P < 0.01 compared with sh-Ctrl group.