Research Paper Volume 14, Issue 1 pp 297—315

TIMP-2 regulates 5-Fu resistance via the ERK/MAPK signaling pathway in colorectal cancer


Figure 5. Knockdown of TIMP-2 overcomes 5-Fu resistance in CRC cells. (A) Changes in expression levels of TIMP-2 in DLD-1 5-FuR cells and HCT116 5-FuR cells after control siRNA or TIMP-2 siRNA (30 pg/ml) transfections. (B, C) Relative cell viabilities of DLD-1 5-FuR cells and HCT116 5-FuR cells under increasing concentrations of 5-Fu for 3 days of culture with TIMP-2 siRNA (30 pg/ml) or TIMP-2 siRNA (30 pg/ml) and recombinant TIMP-2 (10 ng/ml) together. (D) Differences in 5-Fu concentrations for 50% inhibition of cell growth (IC50) between the six groups of cells in Figure 5B and 5C above. Data from triplicate wells for 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test or one-way ANOVA.