Research Paper Volume 14, Issue 1 pp 316—329

Deglycosylated EpCAM regulates proliferation by enhancing autophagy of breast cancer cells via PI3K/Akt/mTOR pathway


Figure 2. Effect of regulators of autophagy on glycosylation of EpCAM breast cancer cells. Treatment of MCF-7 (A) and MDA-MB-231 (B) cells were treated with 100 nM Wortmannin or 200 nM Rapamycin for 12 hr accompanied with transfected with M-EpCAM plasmid. Expressions of autophagy markers Beclin1, LC3, and p62 proteins were determined by western blot analysis.