Figure 4. Transwell assay to investigate the effect of inhibition of hsa_circ_0063526 or/and miR-141-5p on cell invasion of End1/E6E7 cells. (A) si-hsa_circ_0063526 group; (B) si-hsa_circ_0063526 +inhibitor miR-141-5p group; (C) miR-141-5p Inhibitor group; (D) NC-RNA group; (E) Blank control group; (F) The number of cells comparing to the NC-RNA control group. after End1 / E6E7 cell transfected hsa_circ_0063526 siRNA, the cells entering the lower chamber through the pore was lesser compared to the control group, cell invasion ability decreased. After co-transfection with si-hsa_circ_0063526 + inhibitor miR-141-5p, the invasion ability of End1/E6E7 cells was rescued. Replicated 3 times with 3 separate samples (* P<0.05, ** P<0.01) (bar:25μm).