Figure 3. Specific overexpressed and de-methylated genes in CRC tissues were screened by bioinformatics and verified in CRC cell lines by Q-PCR or RT-PCR. (A) Screening specific overexpressed and de-methylated genes in CRC tissues by bioinformatics. (B) Testing the expression of de-methylated genes by Q-PCR in CRC cell lines and a normal colon cell line. The expression of de-methylated genes was tested using Q-PCR (C) and RT-PCR (D) in CRC cell lines after treatment with DAC.