Research Paper Volume 13, Issue 24 pp 26161—26179

Coupling of serum CK20 and hyper-methylated CLIP4 as promising biomarker for colorectal cancer diagnosis: from bioinformatics screening to clinical validation


Figure 4. Serum de-methylated HPDL in CRC, CA patients and healthy controls. (A) Schematic illustration of the gene structure of HPDL, the CpG island region and the position of MSP primers. (B) Detected HPDL methylation status in 8 cell lines. Placental DNA (or treated by SSSI) represented a positive control of de-methylated or methylated status. Abbreviations: M: methylation; U: un-methylation. (C) Representative serum and tissue methylation status of HPDL in CRC, CA patients and healthy controls. (D) Frequency of serum HPDL methylation status in 60 CRC, 30 CA patients and 33 healthy controls.