Research Paper Volume 13, Issue 24 pp 26161—26179
Coupling of serum CK20 and hyper-methylated CLIP4 as promising biomarker for colorectal cancer diagnosis: from bioinformatics screening to clinical validation
Figure 6. Underexpressed and hyper-methylated genes were screened in CRC tissues by bioinformatics and verified in CRC cell lines by Q-PCR or RT-PCR. (A) Screening underexpressed and hyper-methylated genes in CRC tissues by bioinformatics. (B) Testing the expression of hyper-methylated genes by Q-PCR in CRC cell lines and a normal colon cell line. The expression of hyper-methylated genes was tested using Q-PCR (C) and RT-PCR (D) in CRC cell lines after treatment with DAC.