Research Paper Volume 13, Issue 24 pp 26161—26179
Coupling of serum CK20 and hyper-methylated CLIP4 as promising biomarker for colorectal cancer diagnosis: from bioinformatics screening to clinical validation
Figure 7. The serum hyper-methylated status of CLIP4 in CRC, CA patients and healthy controls. (A) Schematic illustration of the gene structure of CLIP4, the position of CpG islands and MSP primers. (B) Detecting CLIP4 methylation status in 8 cell lines. Placental DNA (or treated by SSSI) represented a positive control for de-methylation or methylation. Abbreviations: M: methylation; U: un-methylation. (C) Representative serum and tissue methylation status of CLIP4 in CRC, CA patients and healthy controls. (D) Frequency of serum CLIP4 methylation status in 60 CRC, 30 CA patients and 33 healthy controls.