Figure 3. NAP1L1 controls the expression of cell cycle and apoptosis associated genes via the CCND1/CDK4/CDK6 signaling pathways in glioma. EdU incorporation assay (A), Cell cycle analysis (B), Cell apoptosis assay (C) Mitochondrial membrane potential assay (D) in U87 and LN229 cells transfected with control siRNA (siNC) or NAP1L1 siRNA (siNAP). (E) Western blotting analysis of the protein levels of CCND1, CDK4, CDK6, Bcl-2 and cleaved caspase3 after transfecting siNC or siNAP into U87 and LN229 cells. (F) The H&E of the nude mice tumor tissues. NAP1L1, Ki67, and PCNA was evaluated by immunohistochemical staining. Compared with shNAP cells, the shNC cell tumor tissues were high expression. *P < 0.05, **P < 0.01, ***P < 0.001.