Figure 3. Knockdown of genes for lipid metabolism shortened telomere length (TL) in HepG2 cells. PNPLA2 and CPT1 genes were knocked down by short-hairpin RNA (shRNA) in HepG2 cells. (A) Efficiency of gene knockdown by short-hairpin RNA (shRNA) was evaluated by qPCR. (B) Oil red O staining in HepG2 cells after PNPLA2 and CPT1 knockdown by shRNA. (C) TL was determined by qPCR in HepG2 cells. Data were expression as mean±SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.