Research Paper Volume 14, Issue 1 pp 430—442

Circular RNA circ_0000515 adsorbs miR-542-3p to accelerate bladder cancer progression via up-regulating ILK expression


Figure 2. Knocking down circ_0000515 restrained BC cell multiplication, migration and aggressiveness. After the transfection of siRNA into BC cells: (A) qRT-PCR ensured the enrichment of circ_0000515 and RPPH1 mRNA in total RNA treated with RNase R. (B) qRT-PCR probed expressions of circ_0000515 and RPPH1 with Oligo(dT)18 primers. (C) Circ_0000515 expression in the cytoplasm and nucleus of BC cells was detected by qRT-PCR. (D) qRT-PCR was wielded to probe circ_0000515 expression. (E, F) CCK-8 assay was adopted to monitor the proliferation of BC cells. (G, H) Transwell assay displayed the migrative and invasive capabilities of BC cells. **P<0.01 and ***P<0.001.