Research Paper Volume 14, Issue 1 pp 195—224

Endothelial and systemic upregulation of miR-34a-5p fine-tunes senescence in progeria


Figure 6. miR-34a-5p fine-tunes senescence in progerin-expressing ECs. WT and Prog-Tg lung ECs were transfected with antimiR34a-5p (amir34a-5p) or control antimir67 (amir67) and tested for (A) expression levels of miR34a-5p, and mir34 targets Wnt7a and Sirt1 and (B) Trp53, Cdkn1a and Cdkn2a. WT and Prog-Tg lung ECs were transfected with p53 neutralizing siRNAs or scrambled siRNA (scr) and tested for (C) gene expression levels of senescence markers Trp53, Cdkn1a, Cdkn2a and miR34a-5p and (D) pro-fibrotic markers Ctgf, Edn1 and pro-inflammatory markers (Ccl20, Il1a). (E) BrdU assay performed over 40 h. (F) Schematic representation of miR34a-5p acting on p53- and p16-branch regulating senescence pathways in Prog-Tg ECs. For qPCRs n≥3. Unpaired two-tailed Students t test, ns=non-significant *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.