Figure 5. HFHSD+CUR fed mice maintain normal pancreatic beta-cell integrity. After 15 weeks of diet, (A) pancreas sections from mice on normal chow diet (NCD), curcumin-supplemented normal control diet (NCD+CUR), high-fat high-sugar diet (HFHSD), and curcumin supplemented high-fat high-sugar diet (HFHSD+CUR) were stained for insulin (green) and DAPI (blue). Representative confocal images are shown using a 40X oil objective. Fiji-Image J software (NIH) was used to quantitate (B) total islet size (scale bar, 50 μm), (C) insulin content (mean fluorescence). Quantitative analysis was based on at least 50-70 images per group (n = 3 mice per group). *p ≤ 0.05, ***p ≤ 0.001 and ****p ≤ 0.0001.