Research Paper Volume 14, Issue 1 pp 443—461

PEGylation and antioxidant effects of a human glutathione peroxidase 1 mutant


Figure 7. Effects of GPx1M and SS-mPEG-GPx1M on ADR-induced H9c2 apoptosis evaluated by Hoechst 33258 staining using a fluorescence microscope (200×). The experiments were repeated in triplicate and representative images were shown. Cells were treated with: (A) Control (DMEM). (B) 2.5 μM ADR. (C) and (D) cells preincubated with GPx1M/SS-mPEG-GPx1M (0.08 U/mL) for 1 h, respectively, and then co-incubated with 2.5 μM ADR for 24 h. (E) and (F) cells incubated with 2.5 μM ADR for 12 h, then in co-incubated with GPx1M/SS-mPEG-GPx1M (0.08 U/mL), respectively, for another 12 h. (G) Apoptotic H9c2 cells incubated with different methods. All data were exhibited as mean ± SD. $$$p < 0.001 vs. the control group, #p < 0.05 and ##p < 0.01 vs. the ADR group.