Research Paper Volume 14, Issue 1 pp 443—461

PEGylation and antioxidant effects of a human glutathione peroxidase 1 mutant


Figure 8. Effects of GPx1M and SS-mPEG-GPx1M on ADR-induced generation of ROS in H9c2 cells. Cells were treated with: (A) Control (untreated). (B) 2.5 μM ADR. (C) and (D) cells preincubated with GPx1M/SS-mPEG-GPx1M (0.08 U/mL) for 1 h, respectively, and then in co-incubated with 2.5 μM ADR for 24 h. (E) and (F) cells incubated with 2.5 μM ADR for 12 h, and then co-incubated with GPx1M/SS-mPEG-GPx1M (0.08 U/mL), respectively, for another 12 h. The cells were stained with DCFH-DA and analyzed with a fluorescence microscope (G). The experiments were repeated in triple and results were shown as the mean ± SD. $$$p < 0.001 vs. the control group, #p < 0.05 and ##p < 0.01 vs. the ADR group.