Figure 3. ICH induces neuroinflammation and neuronal loss by activating TLR4-dependent NLRP3 inflammasomes-activating mechanisms in the collagenase injected mice. (A) IL-1β, TNF-α, and IL-6; (B) Iba1, GFAP, and NeuN; (C) NLRP3 and caspase-1; (D) TLR4 and MyD88 protein expression were determined by western blotting analysis. GAPDH served as an internal control. The band densities were measured by Image J to estimate protein levels. The results represent the mean ± SD for the repeated experiments. *P < 0.05; **P < 0.01; ***P < 0.001 vs. sham animals.