Research Paper Volume 14, Issue 1 pp 462—476

Atorvastatin suppresses NLRP3 inflammasome activation in intracerebral hemorrhage via TLR4- and MyD88-dependent pathways


Figure 4. Atorvastatin attenuates the effects of ICH on inducing neuroinflammation and neuronal loss. The ICH mice were treated with the indicated concentration of atorvastatin for 7 d. Total proteins were extracted by RIPA buffer. (A) IL-1β, TNF-α and IL-6; (B) NLRP3 and caspase1; (C) TLR4 and MyD88; and (D) Iba1, GFAP and NeuN protein expression were determined by western blotting analysis. GAPDH served as an internal control. The band densities were measured by Image J to estimate protein quantities. The results represent the mean ± SD for the repeated experiments. *P < 0.05; **P < 0.01; ***P < 0.001 vs. sham animals. #P < 0.05; ##P < 0.01; ###P < 0.001 vs. ICH only animals.