Figure 6. TLR4 pathway activation participates in the activation of NLRP3 inflammasome in ICH-induced neuroinflammation. (A) In TLR4 knocked-down BV2 cells, (B) IL-1β, TNF-α and IL-6; and (C) NLRP3, ASC and caspase-1 protein expression were determined by western blotting analysis. GAPDH served as an internal control. The band densities were measured by Image J and protein quantities estimated. The results represent the mean ± SD for the repeated experiments. *P < 0.05; **P < 0.01; ***P < 0.001 vs. scrambled siRNA.