Figure 3. Effect of young ECM on the nuclear localization of GAPDH in senescent HDFs. (A) Young (Y-HDFs, PD 16) and senescent (S-HDFs, PD 75) cells were seeded onto cover slides coated with young ECM (Y-ECM) or senescent ECM (S-ECM). After incubation for 5 days, cells were immunostained against GAPDH and analyzed by confocal laser scanning microscopy (magnification, 100×; scale bar, 50 μm). (B) The number of cells with cytosolic GAPDH alone (Cytosol) and cells having nuclear GAPDH with or without cytosolic GAPDH (Nuclear +/− Cytosol) was counted, and the percentage distributions were calculated (n = 18 for total replicates) and plotted as means ± standard deviations. ***p < 0.001 (Cytosol) and ###p < 0.001 (Nuclear +/− Cytosol), compared between Y-ECM and S-ECM in Y-HDFs and S-HDFs.