Priority Research Paper Volume 14, Issue 1 pp 4—27

AMP-activated protein kinase-dependent nuclear localization of glyceraldehyde 3-phosphate dehydrogenase in senescent human diploid fibroblasts


Figure 6. AICAR- and SFM-induced GAPDH translocation in AMPKα-knockout MEFs. (A and B) Normal and AMPKα-null MEF cells (AMPKα1−/−, AMPKα2−/−, and AMPKα1−/−/α2−/−) were cultured in 10% FBS medium for 2 days and then treated with 1 mM AICAR (A) or SFM (B) for 2 days. Cells were immunostained with a GAPDH-specific antibody and analyzed by confocal laser scanning microscopy. The number of cells having nuclear GAPDH with or without cytosolic GAPDH was counted, and the percentage distributions were calculated (n = 10 and 20 for total replicates in A and B, respectively) and plotted as means +/− standard deviations. **p < 0.01 and ***p < 0.001, compared with the normal value in the absence of AICAR or SFM (−), ##p < 0.01 and ###p < 0.001, compared each value in the presence (+) to that in the absence of AICAR or SFM (−).