Figure 7. The AMPK signal transduction pathway is involved in the nuclear translocation of GAPDH in senescent HDFs. GAPDH (G) is present mainly in the nucleus of senescent HDFs and other cells from aged animals. When senescent cells are serum-depleted by incubation with SFM or treated with the AMPK activator AICAR, which may result in AMPK activation, GAPDH translocates completely to the nucleus. AMPK inhibition by LPA, young ECM, CompC and AMPKα-siRNAs prevents basal and SFM- or AICAR-induced nuclear translocation of GAPDH. Taken together, the nuclear accumulation of GAPDH in senescent cells can be altered by the modulation of AMPKα expression and activation.