Figure 2. Aged MuSCs exhibited a larger cell volume than young MuSCs. (A) Top view (XY) and cross-sectional images (XZ, white dotted line) of young and aged MuSCs cultured for 3 days and stained for F-actin filaments (green) and nuclei (red) to quantify cell morphometry. (B, C) Cell spreading area and cell apex-height of MuSCs on matrigel coated glass substrate. (D, E) Aged MuSCs exhibited 18% larger cell volume than young MuSC whereas the nuclear volume did not differ. (F–H) Cell shape descriptors were not different between young and aged MuSCs. (I–N) Correlation between cell apex-height, cell volume and cell area of young and aged MuSCs. Abbreviation: MuSCs: muscle stem cells. Values are mean ± SEM. n = 75 cells (from 3 mice per age group). *Significant effect of age, p < 0.05. Scale bar; 10 μm.