Figure 4. Aging is associated with a decline in the number of focal adhesions in MuSCs. (A) Young and aged MuSCs stained for phospho-paxillin (pPXN; magenta, white circles), F-actin filaments (yellow), and nuclei (blue) after 30 min of static and PFSS treatment. (B) Aged MuSCs illustrated lower number of pPXN clusters than young MuSCs and PFSS treatment did not affect the number of pPXN clusters. (C, D) Correlation between pPXN cluster number and cell attachment area in young and aged MuSCs. (E) pPXN cluster area in young and aged MuSCs after static and PFSS treatment. (F, G) Correlation between pPXN cluster area and cell attachment area in young and aged MuSCs. Young MuSCs, n = 63–74 (from 3 young mice). Aged MuSCs, n = 93–95 (from 3 aged mice). (H) Aged MuSCs exhibited lower gene expression of Ptk2. Young MuSCs, n = 11 (from 4 young mice). Aged MuSCs, n = 9 (from 3 aged mice). Abbreviations: MuSCs: muscle stem cells; PFSS: pulsating fluid shear stress. Values are mean ± SEM. *Significant effect of age, p < 0.05. Scale bar: 10 μm.