Figure 4. Changes in the levels of H3 histones in the hippocampus of aged rats after D+Q treatment. Improved cognitive skills, decreased peripheral inflammation, and changes in neuronal synaptic plasticity are accompanied by changes in H3 methylation profile. Trimethylation of H3K9 (A) and H3K27 (B) relative to the whole H3 in the hippocampus of aged rats after D+Q or VEH treatment measured by Western blot. The data are expressed as means ± SEM normalized to the average of the VEH group, and analyzed using t-test; N = 7, *p < 0.05, **p < 0.01, ***p < 0.001.