Figure 4. SORBS2 interacts with YWHAQ. (A) To better understand β-tubulin, ACTN2, CLTL and YWHAQ, we next performed studies in hESC-derived cardiomyocytes (hESC-CMs). Immunofluorescence revealed a clear colocalization of SORBS2 with β-tubulin, ACTN2, CLTL and YWHAQ in the hESC-CMs. The SORBS2 protein (red) colocalized with β-tubulin (green), and the merged images are shown in yellow. (B) Extracted proteins from the cardiomyocytes ware untreated (input) or precipitated by an anti-Flag or anti-Myc antibody and then separated by 4-12% SDS-PAGE. Co-immunoprecipitation (Co-IP) of the heart tissue showing SORBS2 binding with YWHAQ.