Research Paper Volume 14, Issue 2 pp 892—906

The effect of QiangGuYin on osteoporosis through the AKT/mTOR/autophagy signaling pathway mediated by CKIP-1


Figure 4. QGY mediates the effect of CKIP-1 on autophagy through the AKT/mTOR pathway. (A) Co-IP assay in the osteoblasts and osteoclasts. (B, C) Representative figures and quantitative analysis of protein expression of Atg5 and p62 in the osteoblasts (B) and osteoclasts (C). (D) LC3 immunofluorescence assay in the osteoblasts and osteoclasts. (E) RANKL and OPG content analysis in the osteoblasts. (F) Gene expression levels of CKIP-1, RANKL and OPG in the osteoblasts. The data are presented as the means ± SD (B, C, E, F: n=6; D: n=3). *p<0.05, **p<0.01. One-way ANOVA followed by Tukey's post hoc test.