Research Paper Volume 14, Issue 2 pp 892—906

The effect of QiangGuYin on osteoporosis through the AKT/mTOR/autophagy signaling pathway mediated by CKIP-1


Figure 6. CKIP-1 mediated autophagy on the differentiation and function. (A) Alizarin Red Staining in the osteoblasts. (B) RANKL and OPG content analysis in the osteoblasts. (C) Representative figures and quantitative analysis of protein expression of RUNX2 in the osteoblasts. (D) TRACP activity analysis in the osteoblasts. (E) Gene expression levels of CKIP-1, RANKL and OPG in the osteoblasts. The data are presented as the means ± S.D (n=6). *p<0.05, **p<0.01. One-way ANOVA followed by Tukey's post hoc test.