Research Paper Volume 14, Issue 2 pp 1014—1032

A comprehensive analysis of age-related metabolomics and transcriptomics reveals metabolic alterations in rat bone marrow mesenchymal stem cells


Figure 9. Scd2 over-expression alleviates senescence-associated variations in BMSCs. (A) EGFP expression under the fluorescence microscope (scale bar = 200 μm). (B) Determination of Scd2 protein expression levels by Western blot to demonstrate the transduction efficiency. (C) SA-β-gal staining (scale bar = 100 μm) and quantification of β-gal positive cells. (D) Gene expression of the senescence-related factor p16INK4a. Data indicate the mean ± SD, n = 3. *P < 0.05, **P < 0.01, vs. LV-Vector.