Figure 2. Identification of KB1541 interacting proteins. (A) Chemical structure of biotinylated KB1541. (B) Schematic diagram of immunoprecipitation workflow using biotinylated KB1541. (C) Ion Mobility Tandem Mass Spectrometer (IM-MS) identified 837 interacting proteins with biotinylated KB1541. Selected candidates - ATP synthase 5 alpha, ATP synthase 5 beta subunits and 14–3–3ζ protein are shown. -10 logP: -10 log (p-value), Coverage (%): a percentage of the total protein sequence covered by observed peptides, Avg. Mass (kDa): Average of protein molecular mass. (D) Co-precipitation of biotinylated KB1541 and Myc-tagged candidate proteins (Myc-tagged ATP synthase 5 alpha, Myc-tagged ATP synthase 5 beta subunits or Myc-tagged 14–3–3ζ proteins).