Figure 2. Colonic motility dysfunction of T2DM rats. (A) Distal colonic transit time at 8 w following an injection of STZ in the diabetic and control groups. (B) Distal colonic strips of diabetic and control rats were treated with vehicle (DMSO) or glibenclamide (10 μM). Quantification of maximum contractile force of a circular muscle strip stimulated by 10−4 mol/L ACh. (C) Western blotting analysis of the phosphorylation of MLC in the colon muscle layers. Relative expression was normalized to that of GAPDH. Left panel: Representative image; right panel: Quantitative analysis from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, control group n = 10, T2DM group n = 8.