Figure 6. Altered VF expression induced Kir6.1 upregulation. (A) CSMCs were treated with vehicle (PBS solution) or 0, 50, 100, 200, and 300 ng/mL VF for 24 h. Western blotting analysis of KATP subunit protein levels in all the groups. Relative expression was normalized to that of GAPDH. Left panel: Representative image; right panel: Quantitative analysis from three independent experiments. (B) qRT-PCR analysis of KATP subunit mRNA levels in CSMCs treated with vehicle (PBS solution) or 200 ng/mL VF for 24 h. Relative expression was normalized to that of GAPDH. Left panel: Kir6.1 subunit; right panel: SUR 2B subunit. **P < 0.01, ***P < 0.001.