Figure 5. Overexpression of LAMP1 reversed the inhibitory effect of CTRP3 silencing on OGD/R injury in H9C2 cells. H9C2 cells were transfected with CTRP3 siRNA alone or together with pcDNA-LAMP1 for 24 h and then exposed to OGD/R injury. (A) The protein expression of LAMP1 was examined with Western blotting. (B–D) Cell proliferation was detected by using MTT and EdU assays. (E, F) Cell apoptosis ratio was measured by using flow cytometry. (G–I) The production of ROS, MDA and cTn-I was determined.