Priority Research Paper Volume 14, Issue 3 pp 1068—1086

Senescence-associated tumor growth is promoted by 12-Lipoxygenase


Figure 1. Irradiation enhances growth of experimental tumor colonization. Ten week old C57Bl/6NCr mice (n = 10) were irradiated with 5 daily fractions of 6 Gy (6Gyx5) to the thorax. (A) Lung tissue was collected at 2, 4, and 8 weeks after irradiation. SA-β-Galactosidase activity (blue) was evaluated in frozen lung tissue sections. (B) At 2, 4, 8 weeks after irradiation, 5 × 105 B16F0 cells were injected intravenously via the lateral tail vein. Lung tissue was collected 9 days later to count tumor nodules on the lung surface (C) and to measure the size of tumor foci in the lung (D). Panel (A) scale bar 40 μm, panel (D) scale bar 100 μm. Figure A, C, D: * indicate statistical significance compared to 2 wk data from the same group. *denotes p value <0.05.