Research Paper Volume 14, Issue 4 pp 1729—1742

The elevation of miR-185-5p alleviates high-fat diet-induced atherosclerosis and lipid accumulation in vivo and in vitro via SREBP2 activation


Figure 3. MiR-185-5p mimic inhibited lipid metabolic gene expression in HFD-fed apoE-/- mice. (A, B) GSEA enrichment analysis shows that it is enriched in lipid metabolic signaling pathway. (C, D) co-expression analysis revealed that LDLR and FAS were significantly correlated with SREBP2 in atherosclerosis. (E, G) Western blotting and Q-PCR analysis was conducted to assess the protein levels of LDLR, FAS, and SREBP2, SREBP1, SCD1, HMGCR, NLRP3, IL-1β, TNF-α in the aortic sections of HFD-fed apoE-/- mice. (F) nuclear protein levels of SREBP1 and 2. (H) western blotting analysis to test the protein levels of NLRP3, IL-1β, TNF-α. (*,P <0.05; **, P <0.01).