Research Paper Volume 14, Issue 4 pp 1729—1742

The elevation of miR-185-5p alleviates high-fat diet-induced atherosclerosis and lipid accumulation in vivo and in vitro via SREBP2 activation


Figure 5. Effect of miR-185-5p mimic on lipid accumulation in macrophages. Macrophages were pretreated with ox-LDL stimulation. (A) Oil Red O staining revealed that co-treatment with miR-185-5p mimic and ox-LDL significantly decreased the number of lipid droplets. MiR-185-5p inhibitor markedly increased the number of lipid droplets in macrophages. (B) The microsphere phagocytosis assays revealed that significantly fewer fluorescent microspheres infiltrated into the macrophages in miR-185-5p mimic group. MiR-185-5p inhibitor enhanced the recruitment of microspheres into the macrophages (*,P <0.05; **, P <0.01).