Research Paper Volume 14, Issue 4 pp 1767—1781

The novel lncRNA GPC5-AS1 stabilizes GPC5 mRNA by competitively binding with miR-93/106a to suppress gastric cancer cell proliferation


Figure 6. GPC5-AS1 functions as a sponge of the miR-106a family in GC cells. (A) The results from the miRcode database revealed that GPC5-AS1 has binding sites with the miR-106a family. (B) As represented, miR-93 and miR-106a expression levels were measured by qRT-PCR in SGC-7901 cells transfected with pc-GPC5-AS1. (C) GPC5 expression was analyzed in SGC-7901 cells with pc-GPC5-AS1, miR-93 or miR-106a mimics alone or together. (D, E) Expression levels of miR-93 and miR-106a in GC according to the TCGA database. (F) Correlation analysis of GPC5 and miR-93 expression in GC tissues through starBase. (G) The bioinformatics analysis of miR-93 targeting GPC5 and this relationship was validated by dual-luciferase reporter assay in HEK-293 cells. (H, I) Interaction between miR-106a and GPC5 was verified by bioinformatics and dual-luciferase reporter analysis. (p* < 0.05, p** < 0.01).