Research Paper Volume 14, Issue 4 pp 1782—1796

Long non-coding RNA Linc00205 promotes hepatoblastoma progression through regulating microRNA-154-3p/Rho-associated coiled-coil Kinase 1 axis via mitogen-activated protein kinase signaling


Figure 7. miR-154-3p directly targets ROCK1. (A) A schematic representation of putative miR-154-3p binding site in ROCK1. (B) Relative luciferase activity was examined in miR-154-3p and pGL3-ROCK1-wt or pGL3-ROCK1-mut co-transfected cells. (C, D) qRT-PCR and western blot assays for ROCK1 mRNA (C) (n = 60) and protein expression (D) (n = 8) in HB tissues and para-cancerous tissues.*p < 0.05, ***p < 0.001.