Figure 3. Targeting relationship between miR-223-3p and MEK/ERK. (A) The miRNA target prediction databases exposed three potential genes (PLAGL2, FLOT2, and MAP2K1) of miR-223-3p. (B) The qPCR revealed that MAP2K1(MEK1) expression was noticeably decreased in macrophages transfected with miR-223-3p mimics. (C) miRDIP and starBase databases showed that miR-223-3p and MAP2K1 had targeted binding regions. (D) The hierarchical clustering analysis exhibited the distinction on differentially expressed genes in the heat map. (E) Go enriched up-regulated pathways. (F) Go enriched down-regulated pathways. (G) The relevant partial results for KEGG pathways. (F) The gene set enrichment analysis (GSEA) revealed that the innate immune pathway regulation was functionally enriched in AS. (H, I) GSEA revealed that the innate immune pathway regulation was functionally enriched in AS.