Research Paper Volume 14, Issue 4 pp 1865—1878

miR-223-3p inhibits the progression of atherosclerosis via down-regulating the activation of MEK1/ERK1/2 in macrophages


Figure 4. Regulation of inflammatory cytokine secretion and p-ERK1/2 by miR-223-3p. (A) Double-immunofluorescence revealed significant co-localization of the macrophage marker CD68+ and TNF-α in carotid tissues from 223-3p mimics and. (B) The western blots analysis revealed significantly decreased expression levels of IL-6, TNF-a, and p-ERK1/2 in carotid tissues from the miR-223-3p mimics group. The quantitative analysis of relative protein expression exposed the associated decrease in the protein levels of IL-6, TNF-a, and ERK1/2 in carotid tissues of miR-223-3p mimics (*P<0.05).