Figure 5. miR-942 directly targets BARX2. (A, B) The relative mRNA levels of 9 target genes of miR-942 in T24 cells transfected with si-NC or si-circSHPRH. (C–E) The effect of miR-942 overexpression or circSHPRH silencing on BARX2 expression in BCa cells, as detected by western blot. (F) Correlation between miR-942 and BARX2 mRNA expression in BCa tissues. (G) The putative sequences by which miR-942 bound to circSHPRH and the 3’-UTR of BARX2, as predicted by CircInteractome and TargetScan. (H) Luciferase activity was detected in T24 and UM-UC-3 cells transfected with the wild-type (wt) BARX2-3’-UTR (or mutant) and miR-942 mimics or mimics-NC.