Research Paper Volume 14, Issue 4 pp 1941—1958

Fusobacterium nucleatum promotes liver metastasis in colorectal cancer by regulating the hepatic immune niche and altering gut microbiota


Figure 5. Fusobacterium nucleatum–induced intestinal microbial dysbiosis is involved in the development of colorectal cancer liver metastasis. (AD) The rarefaction curve (A), relative abundance curve (B), Shannon diversity index (C), and Chao1 diversity index (D) of intestinal bacteria in mice treated with F. nucleatum or PBS were assessed by 16S rRNA sequencing (n=5 per group). Data are presented as mean ± standard deviation (SD; unpaired Student’s t-test). (E, F) The cecum and livers of mice were dissociated and weighed. The relative cecum weight (cecum weight/body weight) (E) and the metastatic liver weight/cecum weight (MLW/CW) ratio (F) were measured. Data are presented as mean ± SD (n=15 per group). *P < 0.05, **P < 0.01, and ***P < 0.001; unpaired Student’s t-test.