Research Paper Volume 14, Issue 4 pp 1941—1958

Fusobacterium nucleatum promotes liver metastasis in colorectal cancer by regulating the hepatic immune niche and altering gut microbiota


Figure 6. Gut bacterial composition and community structure is altered after Fusobacterium nucleatum administration (A) Principal component analysis was performed on 16S rRNA sequencing of stool samples from mice treated with either F. nucleatum or PBS (n=5 per group). Significant differences (P< 0.05) in intestinal microbiota composition between the two groups were analyzed via PERMANOVA (adonis). (BD) The beta diversity of intestinal bacteria was compared by non-metric multidimensional scaling analysis (B) and weighted (C) and unweighted (D) UniFrac distance analysis between PBS- and F. nucleatum–treated groups. (E, F) The alteration of intestinal bacterial patterns at the phylum level (E) and genus level (F) in mouse stool from the PBS and F. nucleatum groups was assessed via 16S rRNA sequencing (n=5 per group). (G) Specific phylotypes of gut bacteria in the two groups using LEfSe. The histogram shows the LDA scores > 3.5 at the operational taxonomic unit level. (H) The top four genera with the largest difference between the two groups of mice treated with either F. nucleatum or PBS.